{"id":828,"date":"2022-10-11T18:46:46","date_gmt":"2022-10-11T18:46:46","guid":{"rendered":"https:\/\/en.copharm.uobaghdad.edu.iq\/?p=828"},"modified":"2022-10-11T18:46:46","modified_gmt":"2022-10-11T18:46:46","slug":"the-faculty-of-pharmacy-discussed-the-phytochemical-investigation-of-some-active-components-in-iraqi-carica-papaya","status":"publish","type":"post","link":"https:\/\/en.copharm.uobaghdad.edu.iq\/?p=828","title":{"rendered":"The Faculty of Pharmacy discussed the &#8220;Phytochemical Investigation of Some Active Components in Iraqi Carica papaya&#8221;"},"content":{"rendered":"<p style=\"text-align: justify;\"><span style=\"font-size: 19px;\">The Faculty of Pharmacy discussed the master&#8217;s thesis entitled &#8220;Phytochemical Investigation of Some Active Components in Iraqi <em>Carica papaya<\/em>&#8221; by Sura Basim Ghadban and her supervisor, Ass. Prof. Dr. Ali Abdulhussain Kasim, in the Department of Pharmacognosy and Medicinal Plants.\u00a0The study aimed to investigate the phytochemical constituents of Iraqi <em>C. papaya<\/em> leaves and stems and to evaluate the cytotoxic activity of the leaves extract.\u00a0In order to investigate the phytochemical constituents of <em>C. papaya<\/em> leaves and stems extracts, each of the studied parts of the plant was extracted by hot method by soxhlet apparatus using 85% ethanol and fractionation was done by separating the active constituents according to the differences in their polarities using n-hexane, chloroform, ethyl acetate and n-butanol.\u00a0Preliminary identification of different active constituents was performed by specific chemical tests, thin layer chromatography (TLC), high-performance liquid chromatography (HPLC), and gas chromatography\/ mass spectrometry (GC\/MS). A qualitative and quantitative assessment of the number of selected compounds was performed using HPLC analysis.\u00a0Depending on the results of TLC and HPLC, the study revealed the presence of \u03b2-sitosterol, stigmasterol, carpaine, caffeic acid, p-coumaric acid, catechin, apigenin, ferulic acid, quercetin ,kaempferol,mycetrin, gallic acid and chlorogenic acid in the different fractions of <em>C. papaya<\/em> leaves and stems extracts.\u00a0Isolation of active constituents was done using preparative thin layer chromatography (PLC) and HPLC. The isolated compounds were identified using analytical TLC, analytical HPLC, Fourier-transform infrared spectroscopy (FT-IR), ultraviolet (UV) spectroscopy, high-performance compact mass spectrometer (CMS). This study confirmed the identity of the isolated compound that were \u03b2-sitosterol, carpaine, kaempferol, p-coumaric acid, caffeic acid, ferulic acid in different fractions of the <em>C. papaya<\/em> leaves and stems extracts.An unknown compound that was isolated from the n-hexane fraction of the leaves extract by PLC was analyzed to determine the structure of <strong>(<\/strong>S4<strong>)<\/strong>. FT-IR, UV spectroscopy, and nuclear magnetic resonance (NMR) analysis were performed; the unknown compound was proposed to be squalene.\u00a0<\/span><span style=\"font-size: 19px;\">The relative concentration of phytochemical constituents of the different fractions of <em>C. papaya<\/em> was different between the leaves and the stems extracts.\u00a0<\/span><span style=\"font-size: 19px;\">The GC\/MS analysis revealed the presence of a number of compounds that are known to have many biological and pharmacological activities in the n-hexane fraction of <em>C. papaya<\/em> leaves and stem extracts.\u00a0<\/span><span style=\"font-size: 19px;\">Finally, the ethyl acetate fraction of <em>C. papaya<\/em> leaves showed significant cytotoxic activity that was determined using MTT assay, against breast cancer (MCF-7) and lung cancer (A549) cells.\u00a0<\/span><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-831 size-large\" src=\"https:\/\/en.copharm.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/80\/2022\/10\/13-1024x768.jpg\" alt=\"\" width=\"1024\" height=\"768\" srcset=\"https:\/\/en.copharm.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/80\/2022\/10\/13-1024x768.jpg 1024w, https:\/\/en.copharm.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/80\/2022\/10\/13-300x225.jpg 300w, https:\/\/en.copharm.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/80\/2022\/10\/13-768x576.jpg 768w, https:\/\/en.copharm.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/80\/2022\/10\/13-600x450.jpg 600w, https:\/\/en.copharm.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/80\/2022\/10\/13.jpg 1280w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-829 size-large\" src=\"https:\/\/en.copharm.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/80\/2022\/10\/11-1-768x1024.jpg\" alt=\"\" width=\"768\" height=\"1024\" srcset=\"https:\/\/en.copharm.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/80\/2022\/10\/11-1-768x1024.jpg 768w, https:\/\/en.copharm.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/80\/2022\/10\/11-1-225x300.jpg 225w, https:\/\/en.copharm.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/80\/2022\/10\/11-1-600x800.jpg 600w, https:\/\/en.copharm.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/80\/2022\/10\/11-1.jpg 960w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-830 size-large\" src=\"https:\/\/en.copharm.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/80\/2022\/10\/12-1024x768.jpg\" alt=\"\" width=\"1024\" height=\"768\" srcset=\"https:\/\/en.copharm.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/80\/2022\/10\/12-1024x768.jpg 1024w, https:\/\/en.copharm.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/80\/2022\/10\/12-300x225.jpg 300w, https:\/\/en.copharm.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/80\/2022\/10\/12-768x576.jpg 768w, https:\/\/en.copharm.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/80\/2022\/10\/12-600x450.jpg 600w, https:\/\/en.copharm.uobaghdad.edu.iq\/wp-content\/uploads\/sites\/80\/2022\/10\/12.jpg 1152w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Faculty of Pharmacy discussed the master&#8217;s thesis entitled &#8220;Phytochemical Investigation of Some Active Components in Iraqi Carica papaya&#8221; by &#8230; <a class=\"cz_readmore\" href=\"https:\/\/en.copharm.uobaghdad.edu.iq\/?p=828\"><i class=\"fa czico-132-arrows-8\" aria-hidden=\"true\"><\/i><span>more<\/span><\/a><\/p>\n","protected":false},"author":9,"featured_media":831,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_lmt_disableupdate":"","_lmt_disable":"","footnotes":""},"categories":[3,7],"tags":[],"class_list":["post-828","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-latest-news","category-postgraduate-studies-discussions"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.7 - 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