The College of Pharmacy discussed the MSc thesis entitled “Possible Cardio-protective Effects of TND1128 and Omega-3 Fatty Acids against Daunorubicin-Induced Cardiotoxicity in Male Wistar Rats” by the student Huda Hameed Rasheed and the supervisor, Assistant Professor Dr Alaa Radhi Khudhair, at the Pharmacology and Toxicology Department.
The study aimed to evaluate the potential protective effect of both TND1128 and omega-3 against daunorubicin-mediated cardiotoxicity in rats.
The study included 36 male rats, divided into six groups of six each, and treated as follows: Group I rats received 4 mL/kg of corn oil orally for 14 consecutive days; Group II rats received 12 mg/kg daunorubicin for the last 3 days by intraperitoneal injection; Group III rats received omega-3 fish oil at a dose of 600 mg/kg/day orally for 14 consecutive days and injected intraperitoneally with 4 mL/kg normal saline for the last three days; Group IV rats received omega-3 fish oil at a dose of 600 mg/kg/day orally for 14 consecutive days and injected intraperitoneally with 12 mg/kg daunorubicin for the last three days; Group V rats received TND1128 at a dose of 10 mg/kg/day orally for 14 consecutive days and injected intraperitoneally with 4 mL/kg normal saline for the last three days; Group VI rats received TND1128 at a dose of 10 mg/kg/day orally for 14 consecutive days and injected intraperitoneally with 12 mg/kg daunorubicin for the last three days. Subsequently, the rats were sacrificed, and blood and cardiac tissue samples were collected for biochemical, gene expression, and histological analysis.
The results showed improved cardiac tissue in rats pretreated with omega-3 or TND1128, along with a decrease in serum cardiac biomarkers, including troponin I and CK-MB, compared to group II. Also, pretreatment with omega-3 or TND1128 mitigated daunorubicin-induced oxidative stress by increasing GSH and decreasing MDA levels in cardiac tissue. Furthermore, pretreatment with omega-3 or TND1128 reduced daunorubicin-induced inflammation and apoptosis by lowering NF-κB levels and the gene expression of both TNF-α and IL-13, as well as reducing caspase-3 levels in cardiac tissue.
The study recommends further research using larger numbers of animals and longer durations to more comprehensively investigate the protective effects of both TND1128 and omega-3 fatty acids against daunorubicin-induced cardiotoxicity and their potential therapeutic applications.
