The College of Pharmacy discussed the PhD dissertation entitled “The Improving Effects of Different Doses of L-Carvone in Male Mice with Glycerol-Induced Renal Injury Through Rhabdomyolysis-Mediated Effects”, by the student Abeer Riyadh Abdali and the supervisor, Professor Dr. Sarmed Hashim Kadhim, at the Pharmacology and Toxicology Department.
The study aimed to investigate the effects of different doses of L-Carvone on male mice with glycerol-induced nephropathy via rhabdomyolysis-mediated mechanisms.
The study included fifty male mice randomly divided into five groups of 10 and treated as follows: Group I mice received corn oil orally at a dose of 1 mg/kg for five consecutive days and an intramuscular (IM) injection of normal saline at a dose of 10 mg/kg on day five; Group II mice received an IM injection of 50% glycerol at a dose of 10 mg/kg; Group III mice received L-carvone orally at a dose of 25 mg/kg for five consecutive days and an IM injection of 50% glycerol at a dose of 10 mg/kg on day five; Group IV mice received L-carvone orally at a dose of 50 mg/kg for five consecutive days and an IM injection of 50% glycerol at a dose of 10 mg/kg on day five; Group V mice received L-carvone orally at a dose of 100 mg/kg for five consecutive days and an IM injection of 50% glycerol at a dose of 10 mg/kg on day five. On day six, the mice were sacrificed, and blood and kidney tissue samples were collected for biochemical, gene expression, and histological analysis.
The results showed that treatment with L-carvone significantly reduced the histological changes induced by glycerol in renal tissue and lowered serum levels of BUN, creatinine, myoglobin, and CK-MM, as well as KIM-1 and NGAL levels in renal tissue samples. Furthermore, pretreatment with L-carvone alleviated glycerol-induced inflammation and apoptosis by downregulating the expression of TNF-α, IL-1β, and NF-κB, reducing the levels of BAX, caspase-8, and caspase-3, and increasing Bcl-2 levels in renal tissues.
The study recommended conducting further research using different doses of L-carvone over longer periods to evaluate the protective efficacy of this compound against nephrotoxicity and to investigate its molecular mechanisms in greater depth.
