The Faculty of Pharmacy discussed the master’s thesis” Evaluation of the Pharmacological Effect of Iraqi Ammi Majus Alcoholic Extract on Ovalbumin-Induced Asthma in Mice” by Zainab Thanoon Younis and her supervisor, Assist. Prof. Dr. Shihab Hattab Mutlag, in the Department of Pharmacology and Toxicology. This study aimed to evaluate the pharmacological effect of two doses of Ammi majus alcoholic extract for the treatment of asthma induced by ovalbumin in mice. The thesis included Forty-eight albino female mice weighing 25–30 gm were randomly divided into six groups, with 8 mice in each group: Group I: Control group received distilled water for 14 successive days; Group II: Model group (ovalbumin group) received ovalbumin (10%% intraperitoneal injection at day 0 and 1% inhalation for 14 successive days); Group III: Treated group with Ammi majus extract at 64 mg/kg/day (with sensitization) for 14 successive days; Group IV: Treated group with Ammi majus extract at 128 mg/kg/day (with sensitization) for 14 successive days; Group V: treated group with Ammi majus extract at dose (64 mg/kg/day) without sensitization for 14 successive days; Group VI: Treated group with Ammi majus extract at dose (128 mg/kg/day) without sensitization for 14 successive days. After the end of the experiment, all mice were sacrificed by diethyl ether, and blood samples were collected by the retro-orbital route and used to measure the levels of IL-4, IL-5, IL-33, and IgE in serum. Bronchoalveolar lavage fluid was collected to evaluate total and differential White Blood Cell counts. Also, lung tissue was taken for histopathological analysis. The results showed the treatment of mice with ammi majus alcoholic extract had led to a significant reduction in IL-4, IL5, IL-33, and IgE in serum and in total and differential White Blood cell counts in bronchoalveolar lavage fluid when compared to the ovalbumin group (group II). Furthermore, improve histopathologic events in the lungs of mice treated with Ammi majus alcoholic extract. The effect of Ammi majus alcoholic extract as an anti-asthmatic agent derived from it is anti-inflammatory.
