The faculty of pharmacy discussed the master’s thesis tagged “Phytochemical and cytotoxic study of Cassia glauca L. “Fabaceae” cultivated in Iraq” for the student Shamam Kanaan M. Abdulkareem and her supervisor, Professor Dr. Enas Jawad Kadhim, in the Pharmacognosy and medicinal plants department. The aim of the thesis was to conduct a phytochemical analysis of Cassia glauca Lam. species cultivated in Iraq and assess the cytotoxic activity of Cassia glauca Lam. ethyl acetate extract using the MTT assay on the SK-GT-4 cell line. The second part of the research showed that there is a distinct ability of Cassia glauca ethyl acetate fraction to suppress the growth of cancer cell lines and, importantly, this effect is concentration dependent. The results demonstrate the isolation, identification and quantification of seven compounds (stigmasterol, β-sitosterol, quercetine, rutin, isorhamnetin, luteolin, and chlorogenic acid) by using different techniques (TLC, ATR/FTIR UV, HPLC, and LC/MS/MS/Q /TOF).