Faculty of Pharmacy/ University of Baghdad discussed the master’s degree study titled “Phytochemical Investigation of Euonymus japonicus (Family: Cealstraceae) cultivated in Iraq and Evaluation of its Antioxidant and Antimicrobial Activity” by Rasha Abdulrida khalaf and Lecturer. Dr. Thukaa Zuhair Abdul-Jalil in the Pharmacognosy and Medicinal Plants Department. This study aimed to extract and investigate the phytochemicals that are present in the leaves and roots of Euonymus japonicus, then isolate them and study their pharmacological effectiveness as an antioxidant and antimicrobial using plant extracts. The study involved extracting two parts of the plant (leaves and roots) which had not been previously studied using two methods. The crude extracts were collected and dried to undergo various preliminary analyses to examine their phytochemical contents by phytochemical tests, thin layer chromatography (TLC), gas chromatography/mass spectrometry (GC/MS), and high-performance liquid chromatography (HPLC).Many of the active compounds from these parts were isolated by HPLC and PLC, and then the isolated compounds were identified and characterized by thin-layer chromatography (TLC), Fourier transformation of infrared spectra (FTIR), melting point, and ultraviolet-visible spectrophotometry. The result proved the presence of phytochemical compounds (stigmasterol, apigenin, rutin, quercetin, quercetrin, Kaempferol, hesperidin, Naringenin, vitexin, betasitosterol , stigmasterol ,epifriedelanol , p-coumaric acid, and luteolin) that were identified, isolated and purified. The results of the antioxidant activity study were good for ethyl acetate and n-butanol extracted from the leaves by the hot method (Soxhlet). The first study of Euonymus japonicus found that the leaves and roots had good antimicrobial activity against two types of bacteria: gram-positive (Staphylococcus aureus, Streptococcus pneumoniae, Actinomyces naeslundii) and gram-negative (E. coli, Morganella morganii), and one fungal (candida albicans) as compared to the antibiotic cefixime, Augmentin and ketoconazole .
