The College of Pharmacy discussed the master’s thesis entitled Synthesis, Diagnosis, and Evaluation of the Primary Biological Activity of Mannich Base Derivatives from Isatin and 5-Fluoroisatin, Schiff Base Derivatives, by the student Mustafa Mahmoud Mukhlif and his supervisor, Assistant Professor May Muhammad Jawad Al-Mudhafar in the Pharmaceutical Chemistry Department. The thesis aimed to synthesize twelve different compounds of isatin and 5-fluoroisatin by linking them to different secondary amines in the Mannich reaction to obtain the intermediate compounds, which enter the second step of the reaction by linking them to phenylhydrazine in a Schiff base reaction to obtain the final compounds as follows: The first step is to prepare twelve different intermediate compounds by condensing the secondary amines with isatin and 5-fluoroisatin using formalin at a concentration of 37%, where tetrahydrofuran is used as a solvent. The second step is to prepare the final compounds, using the compounds resulting from the first step and their reactant, phenylhydrazine, using a condenser in the presence of glacial acetic acid as a catalyst for the reaction and absolute ethanol as a solvent. The actives were monitored using thin-layer chromatography to ensure the completion of the reactions and the purity of the resulting compounds. The final compounds were examined using the melting point of the compound, infrared spectroscopy, and proton nuclear magnetic resonance spectroscopy, and the results showed good agreement with the compounds that were prepared. An evaluation of the biological effectiveness of the final prepared compounds was conducted against two types of gram-positive bacteria and four types of gram-negative bacteria, and the results showed good effectiveness of the compounds compared to the standard compounds (amoxicillin and ciprofloxacin).The biological effectiveness of one type of fungus was also evaluated, and the results showed strong effectiveness compared to the standard compound flucon
