The Faculty of Pharmacy discussed the master’s thesis entitled” effects of fisetin and cyclophosphamide alone or in combination on hematological parameters, biochemical markers, and genotoxic indices on the bone marrow and spleen of male rats “by Amani Jabbar Hawi and her supervisor, Prof. Dr. Nada Naji Alshawi, in the pharmacology and toxicology department. The study aimed to investigate the effects of fisetin alone and with cyclophosphamide on blood parameters, oxidative stress markers and to reveal its potential genotoxicity or possible protective role against cyclophosphamide-induced genotoxicity in the bone marrow and spleen of male rats. The thesis included Twenty-eight (28) male rats weighing 150-220 gm were randomly-divided into 4 groups where Group I rats received only vehicle (diluted 1% dimethyl sulfoxide solution DMSO) for 7 days; Group II rats received oral fisetin at a dose of (10mg/kg/day) for 7 consecutive days; Group III rats received only a single dose of IP-injection of cyclophosphamide (150mg/kg) 24 hrs. before killing; Group IV rats received oral fisetin at a dose of (10mg/kg/day) dissolved in 1%DMSO for 7 consecutive days; and a single IP injection of cyclophosphamide administered on day 7. Then, after twenty-four (24) hours, animals were IP injected with colchicine at a dose of 3.5mg/kg two hours before euthanization by diethyl ether and scarification by cervical dislocation. From the results obtained from this study, it can be concluded that fisetin alone caused a decrease in white blood cells and thrombocytopenia, which became more intense when it was used with cyclophosphamide, but it has an antioxidant effect against oxidative stress induced by cyclophosphamide, and fisetin had no genotoxicity on bone marrow and spleen of male rats when used alone, with some protection against cyclophosphamide-induced genotoxicity.The study recommended investigating the effect of fisetin as an antioxidant by the utilization of various -doses and the use of various -animals and –tissues. In addition, to study recommend the other mechanisms such as (apoptosis and anti-inflammatory) that may have a role in possible protective effects of fisetin against bone marrow suppression induced by cyclophosphamide. Furthermore, the study recommended clinical study concerning the use of various doses of fisetin with cyclophosphamide or with other chemotherapeutic drugs.
