The Faculty of Pharmacy, discussed the Ph.D. thesis tagged (Study of Interleukin-10 (-1082A/G and -1091A/G) Gene Polymorphisms and Gene Expression in Type 2 Diabetes Mellitus Iraqi Patients in Relation with Toxoplasmosis) for the student in the Clinical Laboratory Sciences Department, Omar Dawood Salman. The objectives of the thesis were to estimate the frequency of toxoplasmosis infection in T2DM patients and to determine the different genotypes and frequencies of IL-10 allele carriage (at locus-1082 A/G and -1091A/G) in Iraqi T2DM patients (with and without toxoplasmosis). In addition to explore the association of IL-10 gene polymorphisms with T2DM and toxoplasmosis among the patients.The thesis included the use of Sanger’s technique for the purpose of determining the presence or absence of genetic polymorphisms for interleukin-10 in the promotor region of the interleukin-10 gene at loci (-1082A/G and -1091A/G) for patients with type 2 diabetes (T2DM) and comparing them with the results of people without diabetes, then studying the effect of these polymorphisms on the level of the interleukin-10 gene expression by real-time PCR technology, which in turn affects the levels of interleukin-10 in the body and their relationship with toxoplasmosis. Interleukin-10 is one of the most important anti-inflammatory cytokines secreted by immune cells inside the body. A higher incidence of T. gondii infection was found in patients with T2DM than in non-diabetics. Carriers of both -1082 AG + GG and -1091 AG + GG genotypes had a significantly synergistic effect on the risk of T2DM. Also, there is an association between T2DM and a polymorphism of the IL-10 gene at positions -1082A/G and -1091A/G. Patients with toxoplasmosis (non-diabetic) carrying the G allele showed decrease in the risk of developing T2DM.
